In this study we report the development of primers to amplify polymorphic chloroplast simple sequence repeats in the genus Hordeum, which includes cultivated barley (H. vulgare ssp. vulgare) and its wild progenitor H. vulgare ssp. spontaneum. Polymorphic products were amplified in a wide range of Hordeum spp. and intraspecific variation was detected in both cultivated and wild barley. A decreas...

The present study aims at detecting molecular markers, based on RAPD, ISSR and AFLP, for the salttolerant wild barley Hordeum spontaneum as a little is known about its genetic structure and function. Barley is one of the most important cereal crops all over the world. Therefore, the identification of molecular markers for the salttolerant wild species is crucial for the future development of to...

field experiment was conducted in order to determine the effect of wheat seeding rate and time of application of sulfosulfuron for control of hordeum spontaneum in wheat field in 2007 in qom province, iran. the experimental design was completely randomized block with four replications in which treatments were arranged as factorial. the treatments were wheat seed rates at 250, 275 and 300 kg/ha ...

In order to study the variation of nitrate reductase (NR) genes among grass species, gene number, intron size and number, and the heme-hinge fragment sequence of 25 grass species were compared. Genomic DNA cut with six restriction enzymes and hybridized with the barley NAD(P)H and NADH NR gene probes revealed a single NAD(P)H NR gene copy and two or more NADH NR gene copies per haploid genome i...

Hordeum, distributed worldwide in temperate zones, is the second largest genus in the tribe Triticeae and includes diploid, tetraploid, and hexaploid species. We determined, by DAPI staining and flow cytometry, the nuclear DNA content for 35 accessions of the genus Hordeum, from a total of 19 species, including specimens of 2 cultivars and 2 landraces of Hordeum vulgare ssp. vulgare as well as ...

Remains of barley (Hordeum vulgare) grains found at archaeological sites in the Fertile Crescent indicate that about 10,000 years ago the crop was domesticated there from its wild relative Hordeum spontaneum. The domestication history of barley is revisited based on the assumptions that DNA markers effectively measure genetic distances and that wild populations are genetically different and the...